Employing the primers 59TTATCCCTCCTTTAAGTGAGATTCTCACAATTG3′ and 5’TTAAAGGAGGGATAAAGGAGTTATGGGT3′ (designated as YAP139UTRmut).Extraction
Chemexpress Applying the primers 59TTATCCCTCCTTTAAGTGAGATTCTCACAATTG3′ and 5’TTAAAGGAGGGATAAAGGAGTTATGGGT3′ (designated as YAP139UTRmut).Extraction of total RNA and miRNATotal RNA was extracted from tissue samples with TRIzol reagent (Invitrogen); miRNA was extracted making use of…