R TNFR1 (1:1,000) for 1 h. Then, the membrane was incubated with secondary
R TNFR1 (1:1,000) for 1 h. Then, the membrane was incubated with secondary Ab for 1 h at room temperature. Peroxidase conjugated anti-mouse IgM goat monoclonal Ab (1:1,500; Calbiochem) for…